fpps gene expression Search Results


gene exp fdft1 hs00926054 m1  (Thermo Fisher)
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rabbit anti farnesyl pyrophosphate synthetase fdps  (Proteintech)
93
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Primer pairs used in this study.
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gene exp fdps dr03424631 g1  (Thermo Fisher)
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Primer pairs used in this study.
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gene exp fdft1 hs00189506 m1  (Thermo Fisher)
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Primer pairs used in this study.
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rabbit anti farnesyl diphosphate farnesyltransferase 1 fdft1  (Proteintech)
93
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Primer pairs used in this study.
Rabbit Anti Farnesyl Diphosphate Farnesyltransferase 1 Fdft1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp ppara hs00231882 m1  (Thermo Fisher)
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Primer pairs used in this study.
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fpps gene expression  (Addgene inc)
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Primer pairs used in this study.
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squalene synthase cass  (Thermo Fisher)
94
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Primer pairs used in this study.
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gene exp fdps rn00821389 g1  (Thermo Fisher)
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Primer pairs used in this study.
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human hepatic squalene synthase  (Zeneca Ltd)
90
Zeneca Ltd human hepatic squalene synthase
Primer pairs used in this study.
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Image Search Results


Primer pairs used in this study.

Journal: Journal of Dental Sciences

Article Title: Periodontal ligament fibroblasts utilize isoprenoid intermediate farnesyl diphosphate for maintaining osteo/cementogenic differentiation abilities

doi: 10.1016/j.jds.2024.04.025

Figure Lengend Snippet: Primer pairs used in this study.

Article Snippet: The sections were incubated with rabbit IgG (DA1E, Cell Signaling Technologies, Danvers, MA, final concentration 10 μg/mL), rabbit anti-farnesyl diphosphate farnesyltransferase 1 (FDFT1) (13128-1-AP, Proteintech, Rosemont, IL, final concentration 10 μg/mL), rabbit anti-farnesyl pyrophosphate synthetase (FDPS) (16129-1-AP, Proteintech, final concentration 10 μg/mL), and rabbit anti-geranylgeranyl diphosphate synthase 1 (GGPS1) (14944-1-AP, Proteintech, final concentration 10 μg/mL) at 4 °C overnight.

Techniques: Sequencing

The isoprenoid metabolic process is most severely downregulated metabolic pathway in PPARγ-knockdown PDLFs. (A) Gene ontology analysis of the downregulated genes in PDLFs transfected with siRNA for PPARG by comparing with PDLFs transfected with control siRNA. Raw RNA-seq data were obtained from NCBI's Gene Expression Omnibus (GEO) under accession number GSE178607 . (B) PDLF-1 cells were cultured in mineralization-inducing medium for 3 and 6 days in the presence of troglitazone (10 μM) or rosiglitazone (10 μM). The gene expression levels of AKR1C3 , ALDH3A2 , AKR1C1 , and SDC3 , which were categorized in “isoprenoid metabolic process” and identified as the downregulated genes in PPARG-knockdown PDLFs, were evaluated. (C) PDLFs were cultured in mineralization-inducing medium for 6 days in the presence of troglitazone (10 μM) or rosiglitazone (10 μM) and FPP (20 μM) or GGPP (20 μM). ALP activities were normalized by cell numbers. (D) PDLFs were cultured in mineralization-inducing medium for 12 days in the presence of FPP (0, 10, 20 μM), and calcium deposition was visualized and quantified by Alizarin Red S staining. (E) Demineralized 1.5-month-old maxilla sections were stained without the primary antibody or with mouse IgG, α-FDPS, α-GGPS1, or α-FDFT1 antibodies, ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001 indicated significantly higher expression levels compared with those in DMSO-treated PDLFs at each day point (B), non-treated PDLFs in either DMSO, troglitazone, or rosiglitazone treatment group (C), and non-treated PDLFs (D). Tro = troglitazone. Ros = rosiglitazone. P = pulp. D = dentin. Pdl = periodontal ligament tissue. Ab = alveolar bone. Scale bars correspond to 100 μm.

Journal: Journal of Dental Sciences

Article Title: Periodontal ligament fibroblasts utilize isoprenoid intermediate farnesyl diphosphate for maintaining osteo/cementogenic differentiation abilities

doi: 10.1016/j.jds.2024.04.025

Figure Lengend Snippet: The isoprenoid metabolic process is most severely downregulated metabolic pathway in PPARγ-knockdown PDLFs. (A) Gene ontology analysis of the downregulated genes in PDLFs transfected with siRNA for PPARG by comparing with PDLFs transfected with control siRNA. Raw RNA-seq data were obtained from NCBI's Gene Expression Omnibus (GEO) under accession number GSE178607 . (B) PDLF-1 cells were cultured in mineralization-inducing medium for 3 and 6 days in the presence of troglitazone (10 μM) or rosiglitazone (10 μM). The gene expression levels of AKR1C3 , ALDH3A2 , AKR1C1 , and SDC3 , which were categorized in “isoprenoid metabolic process” and identified as the downregulated genes in PPARG-knockdown PDLFs, were evaluated. (C) PDLFs were cultured in mineralization-inducing medium for 6 days in the presence of troglitazone (10 μM) or rosiglitazone (10 μM) and FPP (20 μM) or GGPP (20 μM). ALP activities were normalized by cell numbers. (D) PDLFs were cultured in mineralization-inducing medium for 12 days in the presence of FPP (0, 10, 20 μM), and calcium deposition was visualized and quantified by Alizarin Red S staining. (E) Demineralized 1.5-month-old maxilla sections were stained without the primary antibody or with mouse IgG, α-FDPS, α-GGPS1, or α-FDFT1 antibodies, ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001 indicated significantly higher expression levels compared with those in DMSO-treated PDLFs at each day point (B), non-treated PDLFs in either DMSO, troglitazone, or rosiglitazone treatment group (C), and non-treated PDLFs (D). Tro = troglitazone. Ros = rosiglitazone. P = pulp. D = dentin. Pdl = periodontal ligament tissue. Ab = alveolar bone. Scale bars correspond to 100 μm.

Article Snippet: The sections were incubated with rabbit IgG (DA1E, Cell Signaling Technologies, Danvers, MA, final concentration 10 μg/mL), rabbit anti-farnesyl diphosphate farnesyltransferase 1 (FDFT1) (13128-1-AP, Proteintech, Rosemont, IL, final concentration 10 μg/mL), rabbit anti-farnesyl pyrophosphate synthetase (FDPS) (16129-1-AP, Proteintech, final concentration 10 μg/mL), and rabbit anti-geranylgeranyl diphosphate synthase 1 (GGPS1) (14944-1-AP, Proteintech, final concentration 10 μg/mL) at 4 °C overnight.

Techniques: Knockdown, Transfection, Control, RNA Sequencing, Gene Expression, Cell Culture, Staining, Expressing

Correlation between OCN or COL1A1 and FDPS , GGPS1 , or FDFT1 expression in human clinical periodontal tissue. Human clinical periodontal tissues (18 samples from different patients) were collected, and the correlative expression levels of OCN or COL1A1 with FDPS , GGPS1, or FDFT1 were examined. The high (r > 0.8 or r < −0.8) and weak correlations (0.2 < r < 0.4 or −0.4 < r < −0.2) are indicated by green and orange lines, respectively.

Journal: Journal of Dental Sciences

Article Title: Periodontal ligament fibroblasts utilize isoprenoid intermediate farnesyl diphosphate for maintaining osteo/cementogenic differentiation abilities

doi: 10.1016/j.jds.2024.04.025

Figure Lengend Snippet: Correlation between OCN or COL1A1 and FDPS , GGPS1 , or FDFT1 expression in human clinical periodontal tissue. Human clinical periodontal tissues (18 samples from different patients) were collected, and the correlative expression levels of OCN or COL1A1 with FDPS , GGPS1, or FDFT1 were examined. The high (r > 0.8 or r < −0.8) and weak correlations (0.2 < r < 0.4 or −0.4 < r < −0.2) are indicated by green and orange lines, respectively.

Article Snippet: The sections were incubated with rabbit IgG (DA1E, Cell Signaling Technologies, Danvers, MA, final concentration 10 μg/mL), rabbit anti-farnesyl diphosphate farnesyltransferase 1 (FDFT1) (13128-1-AP, Proteintech, Rosemont, IL, final concentration 10 μg/mL), rabbit anti-farnesyl pyrophosphate synthetase (FDPS) (16129-1-AP, Proteintech, final concentration 10 μg/mL), and rabbit anti-geranylgeranyl diphosphate synthase 1 (GGPS1) (14944-1-AP, Proteintech, final concentration 10 μg/mL) at 4 °C overnight.

Techniques: Expressing

Primer pairs used in this study.

Journal: Journal of Dental Sciences

Article Title: Periodontal ligament fibroblasts utilize isoprenoid intermediate farnesyl diphosphate for maintaining osteo/cementogenic differentiation abilities

doi: 10.1016/j.jds.2024.04.025

Figure Lengend Snippet: Primer pairs used in this study.

Article Snippet: The sections were incubated with rabbit IgG (DA1E, Cell Signaling Technologies, Danvers, MA, final concentration 10 μg/mL), rabbit anti-farnesyl diphosphate farnesyltransferase 1 (FDFT1) (13128-1-AP, Proteintech, Rosemont, IL, final concentration 10 μg/mL), rabbit anti-farnesyl pyrophosphate synthetase (FDPS) (16129-1-AP, Proteintech, final concentration 10 μg/mL), and rabbit anti-geranylgeranyl diphosphate synthase 1 (GGPS1) (14944-1-AP, Proteintech, final concentration 10 μg/mL) at 4 °C overnight.

Techniques: Sequencing

The isoprenoid metabolic process is most severely downregulated metabolic pathway in PPARγ-knockdown PDLFs. (A) Gene ontology analysis of the downregulated genes in PDLFs transfected with siRNA for PPARG by comparing with PDLFs transfected with control siRNA. Raw RNA-seq data were obtained from NCBI's Gene Expression Omnibus (GEO) under accession number GSE178607 . (B) PDLF-1 cells were cultured in mineralization-inducing medium for 3 and 6 days in the presence of troglitazone (10 μM) or rosiglitazone (10 μM). The gene expression levels of AKR1C3 , ALDH3A2 , AKR1C1 , and SDC3 , which were categorized in “isoprenoid metabolic process” and identified as the downregulated genes in PPARG-knockdown PDLFs, were evaluated. (C) PDLFs were cultured in mineralization-inducing medium for 6 days in the presence of troglitazone (10 μM) or rosiglitazone (10 μM) and FPP (20 μM) or GGPP (20 μM). ALP activities were normalized by cell numbers. (D) PDLFs were cultured in mineralization-inducing medium for 12 days in the presence of FPP (0, 10, 20 μM), and calcium deposition was visualized and quantified by Alizarin Red S staining. (E) Demineralized 1.5-month-old maxilla sections were stained without the primary antibody or with mouse IgG, α-FDPS, α-GGPS1, or α-FDFT1 antibodies, ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001 indicated significantly higher expression levels compared with those in DMSO-treated PDLFs at each day point (B), non-treated PDLFs in either DMSO, troglitazone, or rosiglitazone treatment group (C), and non-treated PDLFs (D). Tro = troglitazone. Ros = rosiglitazone. P = pulp. D = dentin. Pdl = periodontal ligament tissue. Ab = alveolar bone. Scale bars correspond to 100 μm.

Journal: Journal of Dental Sciences

Article Title: Periodontal ligament fibroblasts utilize isoprenoid intermediate farnesyl diphosphate for maintaining osteo/cementogenic differentiation abilities

doi: 10.1016/j.jds.2024.04.025

Figure Lengend Snippet: The isoprenoid metabolic process is most severely downregulated metabolic pathway in PPARγ-knockdown PDLFs. (A) Gene ontology analysis of the downregulated genes in PDLFs transfected with siRNA for PPARG by comparing with PDLFs transfected with control siRNA. Raw RNA-seq data were obtained from NCBI's Gene Expression Omnibus (GEO) under accession number GSE178607 . (B) PDLF-1 cells were cultured in mineralization-inducing medium for 3 and 6 days in the presence of troglitazone (10 μM) or rosiglitazone (10 μM). The gene expression levels of AKR1C3 , ALDH3A2 , AKR1C1 , and SDC3 , which were categorized in “isoprenoid metabolic process” and identified as the downregulated genes in PPARG-knockdown PDLFs, were evaluated. (C) PDLFs were cultured in mineralization-inducing medium for 6 days in the presence of troglitazone (10 μM) or rosiglitazone (10 μM) and FPP (20 μM) or GGPP (20 μM). ALP activities were normalized by cell numbers. (D) PDLFs were cultured in mineralization-inducing medium for 12 days in the presence of FPP (0, 10, 20 μM), and calcium deposition was visualized and quantified by Alizarin Red S staining. (E) Demineralized 1.5-month-old maxilla sections were stained without the primary antibody or with mouse IgG, α-FDPS, α-GGPS1, or α-FDFT1 antibodies, ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001 indicated significantly higher expression levels compared with those in DMSO-treated PDLFs at each day point (B), non-treated PDLFs in either DMSO, troglitazone, or rosiglitazone treatment group (C), and non-treated PDLFs (D). Tro = troglitazone. Ros = rosiglitazone. P = pulp. D = dentin. Pdl = periodontal ligament tissue. Ab = alveolar bone. Scale bars correspond to 100 μm.

Article Snippet: The sections were incubated with rabbit IgG (DA1E, Cell Signaling Technologies, Danvers, MA, final concentration 10 μg/mL), rabbit anti-farnesyl diphosphate farnesyltransferase 1 (FDFT1) (13128-1-AP, Proteintech, Rosemont, IL, final concentration 10 μg/mL), rabbit anti-farnesyl pyrophosphate synthetase (FDPS) (16129-1-AP, Proteintech, final concentration 10 μg/mL), and rabbit anti-geranylgeranyl diphosphate synthase 1 (GGPS1) (14944-1-AP, Proteintech, final concentration 10 μg/mL) at 4 °C overnight.

Techniques: Knockdown, Transfection, Control, RNA Sequencing, Gene Expression, Cell Culture, Staining, Expressing

Correlation between OCN or COL1A1 and FDPS , GGPS1 , or FDFT1 expression in human clinical periodontal tissue. Human clinical periodontal tissues (18 samples from different patients) were collected, and the correlative expression levels of OCN or COL1A1 with FDPS , GGPS1, or FDFT1 were examined. The high (r > 0.8 or r < −0.8) and weak correlations (0.2 < r < 0.4 or −0.4 < r < −0.2) are indicated by green and orange lines, respectively.

Journal: Journal of Dental Sciences

Article Title: Periodontal ligament fibroblasts utilize isoprenoid intermediate farnesyl diphosphate for maintaining osteo/cementogenic differentiation abilities

doi: 10.1016/j.jds.2024.04.025

Figure Lengend Snippet: Correlation between OCN or COL1A1 and FDPS , GGPS1 , or FDFT1 expression in human clinical periodontal tissue. Human clinical periodontal tissues (18 samples from different patients) were collected, and the correlative expression levels of OCN or COL1A1 with FDPS , GGPS1, or FDFT1 were examined. The high (r > 0.8 or r < −0.8) and weak correlations (0.2 < r < 0.4 or −0.4 < r < −0.2) are indicated by green and orange lines, respectively.

Article Snippet: The sections were incubated with rabbit IgG (DA1E, Cell Signaling Technologies, Danvers, MA, final concentration 10 μg/mL), rabbit anti-farnesyl diphosphate farnesyltransferase 1 (FDFT1) (13128-1-AP, Proteintech, Rosemont, IL, final concentration 10 μg/mL), rabbit anti-farnesyl pyrophosphate synthetase (FDPS) (16129-1-AP, Proteintech, final concentration 10 μg/mL), and rabbit anti-geranylgeranyl diphosphate synthase 1 (GGPS1) (14944-1-AP, Proteintech, final concentration 10 μg/mL) at 4 °C overnight.

Techniques: Expressing